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A sensitive photoelectrochemical immunoassay of N -methyladenosine based on dual-signal amplification strategy: Ru doped in SiO nanosphere and carboxylated g-C N .

Wang, Haiyan; Qi, Chunling; He, Wenhuan; Wang, Minghui; Jiang, Wenjing; Yin, Huanshun; Ai, Shiyun.
Biosens Bioelectron; 99: 281-288, 2018 Jan 15.
Article in En | MEDLINE | ID: mdl-28779697

Abstract

A novel, simple and sensitive photoelectrochemical (PEC) immunosensing platform was constructed for detecting N -methyladenosine (m A) based on the carboxylated CN and avidin functionalized Ru@SiO (ARS) nanocomposite. Herein, the N -methyladenosine-5'-triphosphate (m ATP) was selected as the detection target molecule, m A antibody was used as recognition unit for m A, the carboxylated g-C N (CN) was not only used as the photoactive substance, but the substrate for the m A antibody immobilization, ARS was synthesized and used as signal amplification unit to improve the photocurrent of CN, Phos-tag-biotin was employed as bridge of m ATP and Ru@SiO through the specific interaction between phosphate group of m ATP and Phos-tag, biotin and avidin, respectively. Under the optimal condition, the fabricated PEC biosensor showed a linear range of 0.01-10nM and high detection sensitivity with low detection limit of 3.23pM for m ATP. Besides, the developed strategy was also successfully applied to evaluate the content of m A in human serum samples.